Table 2 Comparison of analytical ultracentrifugation (AUC) and small-angle X-ray scattering (SAXS) data for CitAP-BsLA samples with (+) and without (−) 1 mM sodium citrate.

From: A combination of mutational and computational scanning guides the design of an artificial ligand-binding controlled lipase

 

sedimentation coefficent (AUC)§

Guinier Radius Rg (SAXS)&

frictional ratio (AUC)§

Dmax (SAXS)&

−

+

−

+

−

+

−

+

[S]

[S]

[nm]

[nm]

[f/f0]

[f/f0]

[nm]

[nm]

Monomer$

3.53

3.61

2.94

2.81

1.15

1.12

10.1

9.7

Dimer$

4.49

5.15

3.37

3.44

1.37

1.25

11.1

11.6

>Dimer

6.65

8.36

n.d

n.d

n.d.

n.d.

n.d

n.d

  1. $Molecular mass: monomer: 38.5 kDa, dimer: 77 kDa; n.d. not detected; &Rg and Dmax were determined from SAXS measurements at 0.5 and 5 mg/mL. At low concentration, the determined parameters primarily inform about the structural properties of the monomer, which is the predominwant population at that concentration. At high concentration the dimer is the prevalent species and the structural parameters inform primarily about the properties of the dimer. Dmax and Rg were determined from the distance distribution in real space. §Values represent the mean of two independent sedimentation velocity runs, with an experimental error below 5%.
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