Figure 1: Flowchart of the method developed to identify extracellular lysine residues. | Scientific Reports

Figure 1: Flowchart of the method developed to identify extracellular lysine residues.

From: Identification of Extracellular Segments by Mass Spectrometry Improves Topology Prediction of Transmembrane Proteins

Figure 1

Isolated cells were labeled with a membrane-impermeable, lysine specific labeling agent (sulfo-NHS-SS-biotin). The membrane fraction was purified, solubilized and digested with different proteolytic enzymes. The modified peptides were isolated on a neutravidin agarose resin, then eluted and sequenced by tandem mass spectrometry. Labeled positions were used as extracellular constraints in the CCTOP topology prediction algorithm.

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