Figure 1: GABA inhibits Agrobacterium-mediated T-DNA transformation. | Scientific Reports

Figure 1: GABA inhibits Agrobacterium-mediated T-DNA transformation.

From: An Agrobacterium tumefaciens Strain with Gamma-Aminobutyric Acid Transaminase Activity Shows an Enhanced Genetic Transformation Ability in Plants

Figure 1

(A) GABA content in intact seedlings and cotyledon segments of Micro-Tom. White and black indicate un-inoculated and inoculated, respectively. Bars indicate the standard deviation (n = 3). Different letters indicate significant differences by Tukey’s test (P < 0.01). (B) Classification of GUS-stained cotyledon explants. GUS-stained tomato cotyledons were categorized based on the stained area, as follows: 20% or more of the area, 10% or more, 5% or more, or less than 5%. The frequency of each category of GUS-stained tomato explants is shown. Bacterial strains exhibiting significant differences (Student’s t-test and Kruskal-Wallis test; P < 0.01, n = 80) are indicated with different letters. WT and RNAi refer to the non-transgenic line and the RNAi-SlGADall line, respectively. In GAD RNAi lines, the expression of GAD1, GAD2, and GAD3 was much lower than in WT.

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