Figure 3: Evaluation of the transformation ability of A. tumefaciens with GabT.

(A) GUS-stained explants of tomato (Micro-Tom). Explants were prepared from 7-day-old seedlings. After 3 days of co-cultivation, the explants were stained. (B) Estimation of T-DNA transfer for A. tumefaciens with GabT in Micro-Tom (tomato). GUS-stained tomato cotyledons were categorized based on the stained area, as follows: 20% or more of the area, 10% or more, 5% or more, or less than 5%. Different letters indicate significant differences according to Student’s t-test or the Kruskal-Wallis test; P < 0.01 (n = 80). (C) Assessment of T-DNA transfer for A. tumefaciens with GabT in ‘Moneymaker’. GUS-stained cotyledons were categorized as follows: 20% or more of the area, 10% or more, 5% or more, or less than 5%. Different letters indicate significant differences according to Student’s t-test and the Kruskal-Wallis test; P < 0.01 (n = 80). (D) Occurrence of T-DNA transformation in E. arundinaceus. The number of GUS-stained spots per 1 g of E. arundinaceus calli was counted for each treatment. The bars indicate the standard deviation (n = 3). Different letters indicate values that were significantly different according to Student’s t-test (P < 0.05). Control: A. tumefaciens GV2260 (pBBRMCS1–5, pIG121-Hm); gabT: A. tumefaciens GV2260 (pBBRgabT, pIG121-Hm).