Figure 2: Rheb1 deletion in myeloid cells inhibited mTORC1 activity in mice.
From: Rheb1 deletion in myeloid cells aggravates OVA-induced allergic inflammation in mice
(a) Representative results of PCR genotyping using DNA from cells of mouse tails. Mice with the genotype Lys-MCre-Rheb1flox/flox were defined as KO and those with Rheb1flox/flox as WT. (b) Western blot analysis of BMDMs from Rheb1flox/flox and LysM-Cre-Rheb1flox/flox mice (n = 3). β-actin level was used as the internal reference. (c) Macrophage phagocytosis detection, arrows showed the uptake of Indian ink (400X). (d) The optical density of neutral red (490 nm) (left) and the percentage of cells with ink particles (%) (right). (e) Western blot analysis of sorted alveolar macrophages in BALF from each group (n ≥ 5). β-actin level was used as the internal reference. (f) Flow cytometric analysis of macrophage percentages in bone marrow. (g) Flow cytometric analysis of macrophage percentages in BALF. (h) Quantification of flow cytometric analysis of different cell types in myeloid cells from BM and BALF (i). n = 5, *P < 0.05; **P < 0.01. (j) IF analysis of lung tissue from WT and KO mice in both the control and asthma groups (Scale bar = 10 μm). (k) Quantitative analysis of F4/80 and pS6 positive cells in IF analysis. n ≥ 3, *P < 0.05; **P < 0.01.
