Figure 5: Rheb1 deletion in myeloid cells promotes M2 but inhibits M1 macrophage polarization.
From: Rheb1 deletion in myeloid cells aggravates OVA-induced allergic inflammation in mice
(a) The mRNA expression levels of TNF-α, iNOS and IL-6 in WT and KO mice were determined by real-time PCR after LPS and IFN-γ stimulation for 6 h. (b) The mRNA expression levels of Arg1, CD206, Fizz1, IL-10 and Ym1 in WT and KO mouse bone marrow-derived macrophages (BMMs) were determined by real-time PCR after IL-4 stimulation for 48 h. (c) Representative results of flow cytometric analysis of IL-4-treated BMMs in WT and KO mice. The bar chart shows quantitative analysis of repeated results of flow cytometric analysis (d). Gene expression data are shown as mean ± SD n ≥ 3; *P < 0.05; **P < 0.01; ***P < 0.001; n.s. not significant.
