Figure 7: Extracellular histones increased the rates of CD41-CD45 complex (platelet/leukocyte aggregate) or CD11b-CD45 positive cells via the increasing of C5a.
Mice were injected with histone or saline (45 μg/g, n = 6 per group). The blood samples were collected at 1 h after injection. The profiles of CD11b-CD45 positive cells and CD41-CD45 complex (platelet/leukocyte aggregates; PLAs) are shown in figure (A to B) or (D to E), respectively. The average rates are also shown in figure (C and F), respectively. The concentration of C5a are shown in figure (G). C5a was not detected in C5 deficient mice. The figures show the experimental means ± SD. **P < 0.01 vs Histone group of C5 deficient, ††P < 0.01 vs Vehicle group of C5 sufficient (Scheffe’s test).
