Figure 6: TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-β stimulus.
From: Fibulin-6 regulates pro-fibrotic TGF-β responses in neonatal mouse ventricular cardiac fibroblasts
scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-β stimulation are subjected to (a) FACS analysis. No difference in the amount of TGFβRI and TGFβRII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-β stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-β treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-β stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-β stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test).
