Figure 7: Mutagenesis screen of serine and threonine residues on TRPM7 with regulatory potential. | Scientific Reports

Figure 7: Mutagenesis screen of serine and threonine residues on TRPM7 with regulatory potential.

From: Mass Spectrometric Analysis of TRPM6 and TRPM7 Phosphorylation Reveals Regulatory Mechanisms of the Channel-Kinases

Figure 7

The indicated Sumo-TRPM7-Kinase mutants were purified from E. Coli, and their kinase activities were assayed in in vitro kinase assays using MBP as a substrate. The kinase assays were performed at 30 °C for 2 min. Proteins were resolved by SDS-PAGE and subjected to Coomassie blue staining. Phosphorylation was detected by autoradiography. Residues highlighted in red are the ones found to be phosphorylated by mass spectrometry.

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