Figure 1: Schematics of pathways in catabolic bacterial microcompartments and the propanediol utilizing GRMs. | Scientific Reports

Figure 1: Schematics of pathways in catabolic bacterial microcompartments and the propanediol utilizing GRMs.

From: In Vitro Characterization and Concerted Function of Three Core Enzymes of a Glycyl Radical Enzyme - Associated Bacterial Microcompartment

Figure 1

(A) In the general model for metabolosome function (2, 3), the signature enzymes are involved in the formation of the aldehyde, which is subsequently oxidized to an acyl-CoA thioester by an acylating aldehyde dehydrogenase (AldDH), or reduced to an alcohol by an alcohol dehydrogenase (ADH). The acyl-CoA is then further converted to an acyl-phosphate by a phosphotransacylase (PTA) and finally to the free carboxylic acid by a kinase (AcK), generating ATP. The NAD+ and CoA required for the AldDH reaction are regenerated within the BMC by the ADH and PTA, respectively. (B) Specific enzymes encapsulated in GRM3: The GRE-type 1,2-propanediol dehydratase and its activating enzyme (dark and light purple, respectively) represent the signature enzymes. Encapsulation peptides are depicted as small beige cylinders. The GRM gene cluster of Rps. palustris is shown below. Genes encoding for enzymes that are involved in the encapsulated metabolic pathway are annotated and colored: aldehyde dehydrogenase (PduP), phosphotransacylase (PduL). Genes encoding different types of shell proteins are colored in dark blue, light blue, and yellow. Ancillary genes encoding proteins and enzymes that are not directly involved in the GRM metabolic pathway are white.

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