Figure 4: Comparison of the effect of the E2·E3BP core and its derived domains on PDC inactivation by PDK1-PDK4.
From: Global view of cognate kinase activation by the human pyruvate dehydrogenase complex
Values on Y axis represent % of the remaining PDC activity. The E2·E3BP core and its C-terminally truncated proteins used for activation of PDK1- PDK4 are presented on the X axis. The time of E1 incubation with PDK1-PDK4 with or without lipoyl domain source was chosen as follows: 210 min for PDK1 (left panel, top); 90 min for PDK2 (right panel, top), and 60 min for PDK3 and PDK4 (left and right panels, bottom). Each experiment was done in triplicate and the standard error of mean (SEM) is shown.
