Figure 6: Binding of PDKs to dapoxyl-labeled L3S’.
From: Global view of cognate kinase activation by the human pyruvate dehydrogenase complex
(Top) Fluorescence titration of dapoxyl-labeled L3S’ by PDK2. The dapoxyl-L3S’ (3 μM) in 30 mM KH2PO4 (pH 7.5) was titrated by PDK2 that was accompanied by the enhancement of the dapoxyl-L3S’ fluorescence intensity. The PDK4 behaves similarly. (Bottom) Fluorescence binding curves for dapoxyl-labeled L3S’ on titration by PDK2 and PDK4. Inset: the Kd values for PDK2-L3S’ and PDK4-L3S’ complexes. Excitation wavelength was at 327 nm and the emission spectra were recorded in the 400–650 nm range. No changes in fluorescence intensity of dapoxyl-L3S’ was observed on titration by PDK1 or PDK3.
