Table 1 Kd values for binding of the E2·E3BP-derived domains to PDK1-PDK4 as detected by fluorescence spectroscopy.

From: Global view of cognate kinase activation by the human pyruvate dehydrogenase complex

Kd (μM)

DANS-L1

DANS-L2S

DANS-L1L2S MS1

DANS-L1L2S MS2

DANS-L3S’

Dapoxyl-L3S’

PDK1

4.43 ± 0.35

17.14 ± 0.94

2.10 ± 0.12

3.62 ± 0.25

No binding

No binding

PDK2

0.91 ± 0.08

1.38 ± 0.03

0.89 ± 0.09

0.64 ± 0.09

0.025 a

2.07 ± 0.06

PDK3

0.62 ± 0.03

1.21 ± 0.06

0.54 ± 0.05

0.396 ± 0.06

0.103 a

No binding

PDK4

0.35 ± 0.06

2.07 ± 0.16

0.27 ± 0.02

0.397 ± 0.03

5.93 ± 1.06

5.02 ± 0.13

  1. aOn binding of PDK2 and PDK3 to DANS-L3S’, quenching of the DANS-L3S’ fluorescence was observed while in others enhancement resulted. See SI Experimental Procedures for details.
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