Figure 2: CD3− CD14+ monocytes are the main IL-10 producers in response to B. pseudomallei.
From: Interleukin 10 inhibits pro-inflammatory cytokine responses and killing of Burkholderia pseudomallei
PBMCs from healthy seropositive individuals were incubated with killed B. pseudomallei for 20 hours and, intracellular IL-10 versus cell surface expression of either CD3 or CD14 detected by flow cytometry. The profile (a) of one representative donor was gated on monocytes (b) and lymphocytes (c). d and e shows the frequency of IL-10 producing CD3−CD14+ monocytes (d) or CD3+ lymphocytes (e) from multiple individuals (n = 4). The data in (d) are presented with the mean and the standard deviation. Statistical significance of killed B. pseudomallei versus medium alone was determined using paired T test; ns, non significant, *p < 0.05, **p < 0.01 and ***p < 0.001.
