Figure 6: Neutralization of IL-10 increases IFN-γ, TNF-α and IL-6 production in individuals with DM.
From: Interleukin 10 inhibits pro-inflammatory cytokine responses and killing of Burkholderia pseudomallei
PBMCs (n = 5) were incubated with 10 μg/ml E. coli LPS, killed B. pseudomallei (30:1 ratio) or medium alone in the presence or absence of anti-IL-10 mAb. IFN-γ (a), TNF-α (b) and IL-6 (c) was measured by ELISA from collected supernatant after 48 hours in vitro. Each symbol represents data from an individual. The values from the same individual in the presence or absence of anti-IL-10 mAb are joined by a line. Statistical significance was determined using paired T test; ns, non significant, *p < 0.05, **p < 0.01 and ***p < 0.001.
