Figure 7: Neutralization of IL-10 increases the killing of B. pseudomallei by healthy individuals and individuals with DM.
From: Interleukin 10 inhibits pro-inflammatory cytokine responses and killing of Burkholderia pseudomallei
PBMCs from healthy individuals (n = 7) (a) and individuals with DM (n = 8) (b) were incubated with medium, live B. pseudomallei at an MOI of 1 in the presence or absence of 3 ug/ml of anti-IL-10 mAb for 6 hours. The number of live B. pseudomallei was assessed by colony forming unit assay. The cultured supernatants of healthy individuals (n = 5) (c) and individuals with DM (n = 8) (d) were assayed for IFN-γ, TNF-α and IL-6 by ELISA. Each symbol represents data from an individual. The values from the same individual in the presence or absence of anti-IL-10 mAb are joined by a line. Statistical significance was determined using paired T test; ns, non significant, *p < 0.05, **p < 0.01 and ***p < 0.001. Of note, cultured supernatants from 5 (c) out of 7 (a) healthy individuals were sufficient to be analyzed for cytokine response.
