Figure 4: Functional analysis of aptamers by filter binding assay.

FAM labelled aptamers were incubated with varying concentrations of RSV or HRV formulated in either PBS or throat swab completed PBS and vacuum blotted onto nitrocellulose membrane. The bound aptamers were detected by fluorescence imaging. The selective binding of all studied aptamers were indicated by the distinctively high signal of RSV spiked samples.