Figure 6: Aptamer (H8) binding to RSV and competitive effect of the F-protein selective palivizumab monitored by fluorescence polarization.

The 0.5 nM Alexa Fluor 488 labelled H8 aptamer shows a polarization of ca. 50 mP that increases drastically in mixture with RSV indicating their binding. No change in the fluorescence polarization was obtained in the presence of a 100 × excess of F-protein selective antibody with respect of the aptamer.