Figure 8: Detection of RSV at clinically relevant concentrations ALPHA.
Throat swab samples were spiked with different amount of RSV and the mixtures were supplemented with biotin labeled aptamer and F protein selective antibody. Fluorescence intensities were measured following addition of streptavidin coated donor beads and protein A acceptor beads. For purely aptamer-based detection RSV, the biotin labeled aptamer was coupled to streptavidin donor and acceptor beads. The modified beads were added to throat swab samples spiked with different amount of RSV and fluorescence intensities were measured. The ratios of sample fluorescence (I) and virus-free background fluorescence (Io) are indicated. Both approaches provided distinctive fluorescence signal differences at clinically relevant RSV concentrations.
