Figure 2: PCa cell lines profiled for REST expression and characterization of EMT and stemness signature. | Scientific Reports

Figure 2: PCa cell lines profiled for REST expression and characterization of EMT and stemness signature.

From: REST is a crucial regulator for acquiring EMT-like and stemness phenotypes in hormone-refractory prostate cancer

Figure 2

(A and B) LNCaP, CWR22Rv1, PC3, and DU145 were examined for REST protein by immunoblotting (A) and REST mRNA by RT-qPCR (B). n = 3. (C) Immunoblotting of protein expression for REST, NE marker SYP, and EMT and stemness markers as indicated in LNCaP and CWR22Rv1 cells. n = 3. (D) mRNA levels of NE and EMT markers and CD44 in LNCaP and CWR22Rv1 cells. n = 2. (E and F) Immunofluorescence micrographs (E) and flow cytometric analysis (F) of CD44 expression in LNCaP and CWR22Rv1 cells. n = 2. (G and H) LNCaP and CWR22Rv1 cells were seeded in transwell (1 × 104) or cultured in defined serum-free sphere medium with growth factors (2 × 104) and measured at 3 and 14 days, respectively. The number of migrated cells was quantified by the average from 5 microscopic fields (G). The number of spheres was calculated (H). Representative images (left) and quantification (right) in both PCa cell lines. n = 3. Data represent means ± SD. **p < 0.01, ***p < 0.001 by Student’s-t test.

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