Figure 1: Global identification of proteins upregulated by SOCS2 depletion.

(A) MEFs were transfected with either a non-targeting (NT) siRNA or three different siRNAs against SOCS2. Cell lysates were prepared 36 hrs after transfection and immunoblotted (IB) with the indicated antibodies. Numbers indicate % remaining protein, SOCS2/β-actin. (B) FACS analysis of MEFs transfected with si-SOCS2 #8. (C) Schematics of the proteomic workflow. (D) Principal component analysis of si-Control, si-SOCS2 #2 and si-SOCS2 #8 samples using LFQ intensities by the Perseus platform (n = 3). (E) Volcano plot of distribution of proteins identified in this study. Upregulated proteins (P-value < 0.05 and Fold change >1.5) reported in the literature to have a direct functional link with NF-κB are annotated. Right panel enlists genes corresponding to the annotated proteins which regulates NF-κB activity. “Fold change” and “unique peptides” are indicated.