Figure 3: MAR increases the ratio of positive colonies and growth speed. | Scientific Reports

Figure 3: MAR increases the ratio of positive colonies and growth speed.

From: Matrix attachment region combinations increase transgene expression in transfected Chinese hamster ovary cells

Figure 3

CHO-K1 cells were added G418 (800 μg/ml) at 48 h after transfection cells were incubated for 14 days until single colonies appeared. The cell colonies number and size was observed and calculated based on either white light (left lane) and fluorescence (right lane) (A). Scale bars: 100 μm; (B) Statistical analysis result of the total, positive colonies number and mean positive ratio (%). These results are the mean values obtained for 3 independent experiments, SEM is indicated (Student’s t test, *,#P < 0.05).

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