Figure 4: FAM83H-AS1 cell location and cell cycle regulation.

(A,B) FAM83H-AS1 is mainly located at nuclear measured by RT-PCR. GAPDH was used as cytoplasmic control and PVT1 as nuclear control. (C,D) FAM83H-AS1 knockdown using siRNA caused G2 arrested in both PC-9 (G2 cells increased from 26.4% to 39.4% after siRNA treatment) and H1650 (G2 cells increased from 7.3% to 16.3% after siRNA treatment) lung cancer cell lines. Right panel is the actual number of cell cycles calculated from left panel.