Figure 5: FAM83H-AS1 regulated MET/EGFR signaling. | Scientific Reports

Figure 5: FAM83H-AS1 regulated MET/EGFR signaling.

From: Overexpression of FAM83H-AS1 indicates poor patient survival and knockdown impairs cell proliferation and invasion via MET/EGFR signaling in lung cancer

Figure 5

(A) Receptor tyrosine kinase phosphorylation antibody array indicated phosphor-MET is the most significantly decreased protein after FAM83H-AS1 knockdown by siRNA (10 nM at 96 h) as compared to NT in PC-9 cell. Phosphor-AKT and ERK1/2 were also decreased. (B) Western blot showing MET, EGFR, AKT and ERK1/2 were decreased after FAM83H-AS1 siRNA treatment on PC-9 and H1650 lung cancer cell lines. (C,D) Quantitative analysis of image in B using Image J software. GAPDH was used as protein loading control (NT = 1). Abbreviation: for protein labeling, p = phosphor protein, t = total protein.

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