Figure 2: MiR-146a negatively regulates ADSC osteogenesis. | Scientific Reports

Figure 2: MiR-146a negatively regulates ADSC osteogenesis.

From: Effects of miR-146a on the osteogenesis of adipose-derived mesenchymal stem cells and bone regeneration

Figure 2

(A) qPCR analyses of osteogenic differentiation related genes Runx2, Osterix, OPN and BSP in ADSCs transduced with Lenti-miR-146a, Lenti-miR-146a inhibitor and Lenti-miR-NC. (B) ALP staining of ADSCs transduced with Lenti-miR-146a, Lenti-miR-146a inhibitor and Lenti-miR-NC on days 7 and 14. (C) The ALP activity in ADSCs transduced with Lenti-miR-146a, Lenti-miR-146a inhibitor and Lenti-miR-NC was semi-quantitatively measured on days 7 and 14. (D) ARS staining of ADSCs transduced with Lenti-miR-146a, Lenti-miR-146a inhibitor and Lenti-miR-NC on days 7 and 14. (E) Semi-quantitative analyses of the extracellular calcium deposition of ADSCs transduced with Lenti-miR-146a, Lenti-miR-146a inhibitor and Lenti-miR-NC on days 7 and 14. #Represents significant differences between the Lenti-miR-146a and Lenti-miR-NC groups; *represents significant differences between the Lenti-miR-146a inhibitor and Lenti-miR-NC groups; #P < 0.05, ##P < 0.01, ###P < 0.001; *P < 0.05, **P < 0.01, ***P < 0.001.

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