Table 2 Primers used for qPCR.
Genes | Accession No. | Forward (5′–3′) | Reverse (5′–3′) | Annealing temperature (°C) | Product size (base pairs) |
|---|---|---|---|---|---|
Runx2 | NM_053470.1 | tcttcccaaagccagagcg | tgccattcgaggtggtcg | 60 | 154 |
OPN | NM_012881.2 | ttggctttgcagtctcctgcgg | aggcaaggccgaacaggcaaa | 60 | 106 |
BSP | NM_012587.2 | tggagatgcagagggcaaggct | agttggtgctggtgccgttga | 60 | 137 |
Osterix | NM_152860.1 | cggcaaggcttcgcatctg | ggagcagagcagacaggtgaact | 60 | 166 |
SMAD4 | NM_019275 | ggctggtcggaaaggattt | ccaggtgagacaacccgctc | 60 | 168 |
JMJD3 | NM_001108829.1 | ccctctgtttcctcgtcatc | caagcccgactgatttcttc | 60 | 176 |
GAPDH | NM_017008.3 | aagaaaccctggaccacccagc | tggtattcgagagaagggaggg | 60 | 187 |
