Table 4 Primers used for production and verification of HRM control plasmids.

From: A novel HRM assay for the simultaneous detection and differentiation of eight poxviruses of medical and veterinary importance

Primers’ ID

5′ → 3′sequence

PCR Product size (bp)

OPV-gp060f-For

CGGATGTAAAGACAATGAATGG

1207

OPV-gp060f-Rev

AAACGATTGACGTCCGAAAT

CaPV-RPO147f-For

TCAATTAACTAGAATAAAGCAAGGAAA

1120

CaPV-RPO147f-Rev

TCCTCTCCCCTCTGGATCTT

PaPV-VLTF3f-For

TCGGCAGCACGTACTCGAT

855

PaPV-VLTF3f-Rev

AGTGCTGGACCGCGAGAT

  1. The sequences of primers used for the amplification, cloning and sequencing of the full ssDNA-binding phosphoprotein gene (I3L) of orthopoxviruses, the partial RNA polymerase subunit gene (RPO147) of capripoxviruses, and the full late transcription factor gene (VLTF3) of parapoxviruses are presented with their estimated PCR product size are presented.
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