Figure 4: PCLSR evaluation using different ASFV genotypes or strains and matrices.

(A) Genotype and strain specificity testing. 1 – CV98 strain (genotype I), 2 – BF07 strain (genotype I), 3 – E70 strain (genotype I), 4 – Arm07 strain (genotype II), 5 – LT14/1490 (genotype II), 6 – Ukr12/Zapo (genotype II), 7 – Estonia 1 (genotype II), 8 – Estonia 2 (genotype II), 9 – Estonia 3 (genotype II), 10 – Estonia 4 (genotype II), 11 – Estonia 5 (genotype II), 12 – Estonia 6 (genotype II), 13 – Estonia 7 (genotype II), 14 – Moz64 (genotype V), 15 – Malawi (genotype VIII), 16 – Ken06.Bus (genotype IX), 17 – Ken08K.2/1 (genotype X), 18 – Ug64 (genotype X). (B) Testing of matrices from infected animals. 1 – bone marrow diluted 1:10 w/v, 2 – bone marrow concentrated, 3 – blood of pig, 4 – blood of pig (clot), 5 – serum of pig, 6 – blood of wild boar, 7 – spleen, 8 – kidney, 9 – lymph node, 10 – tonsil, 11 – lungs, 12 – muscle fibers, 13 – cDNA extracted from a classical swine fever virus (CSFV) strain Alfort/187; 14 – cDNA extracted from a porcine reproductive and respiratory syndrome virus (PRRS) strain Lelystad (genotype I), 15 – positive control standard ASFV plasmid containing a p72 gene fragment (7.2 × 10⁷ copies μl⁻¹), 16 – blood collected from a non-infected pig.