Figure 2: Proteomic characterization of BoNT/C1 ad heterodimer.
From: Engineering Botulinum Neurotoxin C1 as a Molecular Vehicle for Intra-Neuronal Drug Delivery
(A) light chain sequence; (B) heavy chain sequence. Letters on white background represent confirmed sequence identified among one or more corresponding peptides from enzymatic digests of the recombinant protein. Letters on light blue background represent sequences not recovered from any of the digests. Blue letters represent exogenous linkers/spacers introduced into the recombinant protein. Boxed letters in Panel A mark positions of five non-adjacent amino acids – H, E, H, E, Y – that form the active site of Zn2+-metalloprotease; red letters among this group represent mutations E238 > A; H241 > G; Y383 > A introduced into the metalloprotease active site rendering it inactive in this atoxic derivative. Orange letters represent the TEV protease recognition sequence. Green letters represent the two cysteine residues that form the disulfide bridge between light and heavy chains of the heterodimer. Magenta letters (Panel B) represent the HA tag introduced at the C-terminus of the heavy chain for detection purposes.
