Figure 9: After systemic administration, BoNT/C1 ad specifically accumulates at the neuromuscular junction and co-localizes with synaptic proteins. | Scientific Reports

Figure 9: After systemic administration, BoNT/C1 ad specifically accumulates at the neuromuscular junction and co-localizes with synaptic proteins.

From: Engineering Botulinum Neurotoxin C1 as a Molecular Vehicle for Intra-Neuronal Drug Delivery

Figure 9

Six week-old mice were injected ip with 0.4 mg/kg of BoNT/C1 ad. 24 hours after systemic injection, mice were euthanized, and diaphragms were isolated and prepared for staining. Tissue was stained with mAbs raised against Syx1 (pre-synaptic marker), BoNT/C1 HC (probed with anti-human IgG 8DC1.2), and α-bungarotoxin (post-synaptic marker), and analyzed by confocal microscopy. Bar = 10 μm.

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