Table 1 BoNT/C1 ad mouse lethality assay.
From: Engineering Botulinum Neurotoxin C1 as a Molecular Vehicle for Intra-Neuronal Drug Delivery
Determination of mouse LD50 for BoNT/C1 ad used in this study | ||
|---|---|---|
Dose (mg/kg) | Percent survival (n = 10) | Comments on surviving mice, 5 days observation time |
0.04 | 100 | No symptoms for all mice injected. All mice bright, alert, responsive throughout duration of experiment |
0.20 | 100 | No symptoms for all mice injected. All mice bright, alert, responsive throughout duration of experiment |
0.40 | 100 | No symptoms for all mice injected. All mice bright, alert, responsive throughout duration of experiment |
0.80 | 100 | No symptoms for all mice injected. All mice bright, alert, responsive throughout duration of experiment |
2.00 | 90 | 2 mice bright, alert, responsive throughout duration of experiment; 6 mice bright, alert, responsive with low level severity of abdominal breathing; 1 mouse bright, alert, responsive with high level severity of abdominal breathing. 7 mice with symptoms are fully recovered within 72 hours after injection. |
4.00 | 70 | 3 mice bright, alert, responsive throughout duration of experiment; 4 mice bright, alert, responsive with high level severity of abdominal breathing. These 4 mice with symptoms are fully recovered within 72 hours after injection. |
5.00 | 50 | 5 mice bright, alert, responsive with high level severity of abdominal breathing. These 5 mice with symptoms are fully recovered within 72 hours after injection. |
6.00 | 10 | 2 mice bright, alert, responsive with high level severity of abdominal breathing. These 2 mice with symptoms are fully recovered by day 5 after injection. |
