Figure 4: Axially-locked method in hippocampal slices.
From: Image-based adaptive optics for in vivo imaging in the hippocampus
(A) Example ROI, 200 μm deep in a GAD67KI-GFP hippocampal slice, without and with wavefront correction (No AO and Full AO), scale bar: 100 μm. (B) Axial variation of the metric M3 in the same slice, the maximum of the metric was at 198 μm. (C) Axial profiles of a representative neuron without and with wavefront correction (No AO and Full AO) and their respective fit. (D) Signal and resolution increase with wavefront correction, calculated using the axial fit of n = 36 somata; boxplots showing median (red lines) and inter-quartile range (IQR, rectangle), whiskers and outliers are calculated with a 1.5*IQR threshold. (E) Median amplitude of the correction (n = 5 FOVs) on each considered Zernike modes for setup correction and full (setup + sample) correction, Zernike modes are indexed following the A176988 sequence.
