Figure 5: Axially-locked method in living mouse.
From: Image-based adaptive optics for in vivo imaging in the hippocampus
(A) Example ROI, 175 μm deep in the CA1 hippocampal region of a living mouse, without and with wavefront correction (No AO and Full AO), scale bar: 100 μm. (B) Axial variation of the metric M3 in the same slice, the maximum of the metric was at 168 μm, the pyramidal cell layer spanned from 162 to 187 μm. (C) Axial profiles of a representative neuron without and with wavefront correction (No AO and Full AO) and their respective fit. (D) Signal and resolution increase with wavefront correction and setup correction, calculated using the axial fit of n = 11 somata; boxplots showing median (red lines) and inter-quartile range (IQR, rectangle), whiskers and outliers are calculated with a 1.5*IQR threshold. (E) Amplitude of the correction on each considered Zernike modes for setup correction and full (setup + sample) correction, Zernike modes are indexed following the A176988 sequence.
