Figure 2: FISH analysis enables to detect responses of cells against two images presented with 60 min interval.

(a) Experimental design. Chicks were placed in the apparatus for 5 min (1st session), returned to the dark incubator, and after 60 min, they were placed in the apparatus again for 5 min (2nd session). C, exposed to the black screen without any image; R, exposed to the moving red square presented on a black screen. (b) Representative images of Arc expression (green) in the VW of chicks in the indicated conditions. Nuclei are stained (PI, magenta). Gray arrowheads indicate the cells with both nuclear and cytoplasmic transcripts (Nuc + Cyto cells). (c) Note that the percentage of Cyto cells is significantly higher in chicks that were exposed to the moving red square during the 1st session compared to other chicks, and the percentage of Nuc cells is significantly higher in chicks that were exposed to the moving red square during the 2nd session compared to other chicks. The percentage of Nuc + Cyto cells in R–R chicks is significantly higher than that of other chicks. (d) Venn diagrams showing percentages of cells classified according to the Arc expressing pattern. The number is the mean value of 6 samples and the circle size correlates with the magnitude of number. Table shows the percentages of Nuc + Cyto cells to Cyto cells or Nuc cells in each condition. Mean ± SE calculated from 6 samples is shown. (e) Number of cells (gray plus white bar) was not significantly different among groups, but the number of Arc expressing cells differed between groups. **p < 0.01. Scale bar = 20 μm.