Figure 4: Imprinting altered activity of the parvalbumin-positive inhibitory neurons in the IHA, afferent to the HI/HD layers. | Scientific Reports

Figure 4: Imprinting altered activity of the parvalbumin-positive inhibitory neurons in the IHA, afferent to the HI/HD layers.

From: Regulation of visual Wulst cell responsiveness by imprinting causes stimulus-specific activation of rostral cells

Figure 4

(a) Arc Expression and the pathway for visual imprinting in the chick telencephalon. This schema is derived from the same photograph as those presented in Figs 1b and 3c. A black square in the IHA layer encloses the analysed region. (b) Image of immunostaining with anti-parvalbumin (PV) antibody. PV cells are prominent in the IHA layer. The enclosed area indicates the analysed region, which is identical to the region shown in a. (c). Fluorescent staining of PV protein (green) and GAD65 mRNA (magenta). (d) Almost all PV cells (98 ± 0.2%) express GAD65, and almost all GAD65-positive cells (91.0 ± 0.9%) express PV. (e) Fluorescent staining images of Arc transcripts (green) and PV protein (magenta) in RT–R chicks. (f) The percentages of Cyto cells, Nuc cells, and Nuc + Cyto cells among PV cells in RT–R and CT–R chicks were calculated. The percentages of PV-positive Cyto cells and Nuc + Cyto cells in RT–R chicks are higher than those in CT–R chicks. (g) Number of PV cells as well as Arc-positive PV cells in CT–R chicks was not significantly different from those of RT–R chicks. (h) Venn diagrams and table created similarly as Fig. 2d. *p < 0.05; **p < 0.01. Scale bar = 250 μm (a), 100 μm (b), 20 μm (c,e).

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