Figure 9: aSyn prevents mitosphere formation induced by 6-OHDA. | Scientific Reports

Figure 9: aSyn prevents mitosphere formation induced by 6-OHDA.

From: Alpha-synuclein prevents the formation of spherical mitochondria and apoptosis under oxidative stress

Figure 9

(a,b) H4 cells were treated with different concentrations of 6-OHDA (a) or Rotenone (b) for 4 or 14 h and mitochondrial alterations are shown via MT staining. (c) Whereas the “donut-shaped” mitochondrial alterations induced by Rotenone were also frequently found in aSyn H4 cells, aSyn overexpression significantly reduced 6-OHDA-induced mitosphere formation. Quantification of mitospheres under 6-OHDA treatment (200 μM, 4 h) was performed in mixed cultures of aSyn and Ctr H4 cells (unpaired two-tailed t-test, n = 3 with >600 cells counted per sample, p = 0.0086).

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