Figure 2: Relative peptide-spectrum-match (PSM) counts of antibodies and the protein subunits of Pol V complex. | Scientific Reports

Figure 2: Relative peptide-spectrum-match (PSM) counts of antibodies and the protein subunits of Pol V complex.

From: Methylated-antibody affinity purification to improve proteomic identification of plant RNA polymerase Pol V complex and the interacting proteins

Figure 2

The peptides were generated from the immunoprecipitated proteins (“Control 1”: non-methylated antibody, on-bead trypsin digestion; “Control 2”: non-methylated antibody, on-bead digestion with endoproteinase Lys-C and then cleavage by trypsin; “Methylated antibodies”: antibodies modified by dimethylation at lysine residues, on-bead digestion with endoproteinase Lys-C and then cleavage by trypsin). Error bars are standard deviations from triplicate experiments. (A) Relative PSM counts from individual Pol V complex proteins as well as antibody light (L) and heavy (H) chains. Blue, yellow and red columns represent results from “Control 1”, “Control 2” and “Methylated antibody” purification, respectively. Protein identification and PSMs were generated by Mascot database search against TAIR10. (B) The insert is shown the total PSM distribution of antibodies (purple triangles) versus the Pol V complex (green circles), and the dashed line is shown the tendency of the PSM changes.

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