Figure 6: Inhibition assay.
Progressive curve showing hydrolysis of chromogenic substrate T2943 from Sigma CH3SO2-D-HHT-G-R-pNA.AcOH by tPA at 25 °C in the presence of 0 NS (blue line), 100 nM WT NS (black line), 100 nM W154A (green line) and 1000 nM W154P (red line). Inset: enlarged view of first 30 min of reaction. Table showing ka of WT NS, W154A and W154P, with ± standard deviation of at least three independent experiments. *The data for ka was found to be statistically significant with P < 0.001 for both W154A and W154P. One way ANOVA was used for determining statistical significance and P value was determined using t-test.
