Figure 7: Complex formation analysis of WT NS and 154 variants. | Scientific Reports

Figure 7: Complex formation analysis of WT NS and 154 variants.

From: A hydrophobic patch surrounding Trp154 in human neuroserpin controls the helix F dynamics with implications in inhibition and aggregation

Figure 7

Complex formation of (a) WT NS (b) W154A (c) W154P with tPA in 50 mM HEPES buffer [pH 7.4] at 25 °C. Aliquots of reaction were taken at different time intervals from 0 to 180 min. Samples were run on 12% SDS-PAGE. Each lane contains 65 nM NS and 13 nM tPA. The arrow indicates the different species: complex, tPA, native NS and cleaved NS on SDS-PAGE. The band densities from SDS-PAGE (d) WT NS and (e) cleaved NS (WT NS (solid lines), W154A (dashed lines) and W154P (dotted lines)).

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