Figure 3: Internalization of IgM on lymphocyte subsets.
From: Glycan-independent binding and internalization of human IgM to FCMR, its cognate cellular receptor
(a) Gating strategy for lymphocyte subsets known to express hFCMR. PBMCs were labeled with 1 μM LIVE/DEAD Aqua Dead Cell stain, anti-human CD3-Brilliant Violet 421, anti-human CD56-Alexa Fluor 700, anti-human CD8-PerCp, and anti-human CD4-FITC for 1 hr at 4 °C. To differentiate B cells, LIVE/DEAD stained PBMCs were incubated with anti-human CD3-Brilliant Violet 421 and anti-human CD19-FITC. Labeled cells were analyzed and gated by flow cytometry. (b) Surface levels of IgM (GMFI) on gated lymphocytes incubated with media alone (no IgM) or media supplemented with 10% human serum. The mean ± SD of three independent experiments is shown.
