Figure 4: Effect of de-glycosylation on FCMR-mediated IgM internalization.
From: Glycan-independent binding and internalization of human IgM to FCMR, its cognate cellular receptor
PNGase F (a) and neuraminidase (b) were used to cleave glycans from IgM molecules. The binding of endoglycosidase-treated (green trace) and non-treated (black trace) hIgM to hFCMR-transfected cells is shown as traces in the left panels. Internalization of endoglycosidase-treated IgM was also investigated, whereby IgM surface levels (GMFI) were normalized to time 0 (right panels). The mean ± SD of three independent repeats is shown.
