Figure 3: RESV increases HMGB1 in the nucleus by Sirt1-mediated translocation inhibition. | Scientific Reports

Figure 3: RESV increases HMGB1 in the nucleus by Sirt1-mediated translocation inhibition.

From: Resveratrol treatment reveals a novel role for HMGB1 in regulation of the type 1 interferon response in dengue virus infection

Figure 3

Mock-treated, 80 μM RESV-treated and Sirt1-knockdown Huh7 cells were infected with DENV at an MOI of 1. Cells were harvested after 24 h p.i. and the nuclear (A) and cytosolic (B) proteins were determined by Western blot for HMGB1 detection. Statistically significant differences between the groups are indicated: *P < 0.05. (C) The supernatants of the cell culture were harvested and HMGB1 was measured using Western blot. (D) Cells were fixed after 24 h p.i. for immunofluorescence analysis (IFA). Different treatment groups are as indicated. For detection of HMGB1, cells were stained with rabbit anti-HMGB1 and donkey anti-rabbit-IgG Alexa Fluor 594 (Red). For detection of DENV E protein, cells were probed with mouse anti-E protein antibody and goat anti-mouse-IgG Alexa Fluor 488 (Green). The cell nuclei were stained with DAPI (Blue).

Back to article page