Figure 4: Antiviral activity of RESV relies on the presence of HMGB1 and Sirt1. | Scientific Reports

Figure 4: Antiviral activity of RESV relies on the presence of HMGB1 and Sirt1.

From: Resveratrol treatment reveals a novel role for HMGB1 in regulation of the type 1 interferon response in dengue virus infection

Figure 4

(A) Huh7 WT, shHMGB1 and shSirt1 cells were infected with DENV at an MOI of 1. Cells were harvested at 24 h p.i. and DENV NS3, HMGB1 and Sirt1 were detected by Western blot analysis. (B) The supernatants of the cell culture were harvested and virus titers were detected using focus assay. Statistically significant differences between the groups are indicated: *P < 0.05, **P < 0.01. (C,D) Huh7 WT, shHMGB1 and shSirt1 cells were mock-treated or treated with 80 μM RESV after infection with DENV. Levels of DENV NS3, HMGB1 and Sirt1 were analyzed using Western blot. Band intensities for the ratio of DENV NS3 and β-actin were determined by Image J analysis.

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