Figure 1: MS-HRM of the DUSP1 promoter methylation for serials standards and samples.

(A) Normalized HRM curves. The DNA methylation standards of 0 (universal unmethylated DNA), 1, 5, 20, and 100% methylation (universal methylated DNA) are indicated. (B) Tm plot (negative first derivative of the HRM curves) of serials standards. (C) The melting profile of a methylated breast tumour DNA sample (sample 1 with methylation level of 1–5%). (D) The melting profile of an unmethylated tumour DNA sample (sample 2).