Figure 2: Regionally specific terminal cell divisions in the developing utricle. | Scientific Reports

Figure 2: Regionally specific terminal cell divisions in the developing utricle.

From: Establishment of planar cell polarity is coupled to regional cell cycle exit and cell differentiation in the mouse utricle

Figure 2

Pregnant female mice were injected once with 5-ethynyl-2′-deoxyuridine (EdU) to label cells in S phase at embryonic day (E)11.5 (A, A’, A”), E12.5 (B, B’), E13.5 (C, C’), E14.5 (D, D’), and E16.5 (E, E’), and allowed to survive until E18.5. Each sample was stained for EdU to mark the cells in the S phase of the cell cycle at the time of injection, and with an antibody to myosin VIIa for hair cells and OCM for type I hair cells in the striola. Scale bar: 100 μm. Fields in yellow and blue box (A”) were chosen from striola and MES respectively for a higher magnification image. We only counted double labelled cells for statistical analysis. The percentages of hair cells born at each stage in each of the three segments of the utricle, striola, medial extrastriola (MES), and lateral extrastriola (LES) (F). At E13.5, the ratios of EdU and myosin VIIa double-positive hair cells to the total number of hair cells were 0.331 ± 0.089, 0.233 ± 0.016, and 0.219 ± 0.036 in LES, striola, and MES, respectively (P < 0.01, n = 5). At E16.5, the ratios of EdU and myosin VIIa double-positive hair cells to the total number of hair cells were 0.133 ± 0.034, 0.009 ± 0.002, and 0.012 ± 0.004 in LES, striola, and MES, respectively (P < 0.01, n = 5).

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