Figure 5: The cell-intrinsic polarity proteins and core PCP protein expression in the utricle. | Scientific Reports

Figure 5: The cell-intrinsic polarity proteins and core PCP protein expression in the utricle.

From: Establishment of planar cell polarity is coupled to regional cell cycle exit and cell differentiation in the mouse utricle

Figure 5

E11.5 (A), E12.5 (B), E13.5 (C–E,G) and E16.5 (F) mouse utricles were imaged. At E11.5, Vangl2 was expressed in the cell boundaries of utricle before hair cell polarity formation, while prickle2 didn’t emerge (A). At 12.5, cell-intrinsic protein Gαi3 started to appear in the utricle (B). At E13.5 when the LPR began to form, Gαi3 was found in the bare zone of hair cell (C), Pard6B was on the opposite side (D), Vangl2 and Prickle2 located at the same side of hair cell though they were two groups of hair cells positioned at the different sides of the LPR (E). At E16.5, different hair cells were not in the same development stages (F). In early development stage, Gαi3 was located in the whole cytoplasm of hair cell (White box). In later stage, Gαi3 moved to the bare zone (Yellow box). Schematic representation of hair cells in different development stage in E13.5 utricular epithelium (G). Gαi3 was gradually polarized to the bare zone, and kinocilium relocalized from centre to one side of hair cell. Fields in white or yellow box were chosen for a higher magnification image. Scale bars: 10 μm (A–F), 5 μm (G).

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