Figure 1: Gel-electrophoresis, SDS-PAGE and Western blotting analysis of mt-PA. | Scientific Reports

Figure 1: Gel-electrophoresis, SDS-PAGE and Western blotting analysis of mt-PA.

From: Scale up and pharmacokinetic study of a novel mutated chimeric tissue plasminogen activator (mt-PA) in rats

Figure 1

(a) Gel-electrophoresis analysis of the mt-PA- pTracer-SV40 Plasmid by SmaI restriction enzyme digestion on 1% agarose gel. (Lane 1) DNA Ladder 1 Kb. (Lane 2) pTracer-SV40 Plasmid without the mt-PA gene. (Lane 3) pTracer-SV40 Plasmid without the mt-PA gene digested by SmaI restriction enzyme. (Lane 4) pTracer-SV40 Plasmid with the mt-PA gene. (Lane 5) pTracer-SV40 Plasmid with the mt-PA gene digested by SmaI restriction enzyme. (b) The mt-PA expression analysis in transfected Expi293F cells supernatant on a 12% SDS-PAGE gel. (Lane 1) Supernatant from non-transfected Expi293F cell culture medium as negative control. (Lane 2) The mt-PA Expi293F cell culture medium. (Lane 3) Rainbow Molecular Weight Marker (code RPN756). (Lane 4) Alteplase as positive control. (c) Western blotting detection of the mt-PA expression in the supernatant of transfected Expi293F cells. (Lane 1) Supernatant from non-transfected Expi293F cell culture medium (negative control). (Lane 2) The mt-PA Expi293F cell culture medium. (Lane 3) Rainbow Molecular Weight Marker (code RPN756). (Lane 4) Alteplase (full length form). The samples in a (gel) and b (blot) derived from the same experiment and the gel and blot were processed in parallel.

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