Figure 2: The analysis of the affinity chromatography fractions of the transfected Expi293F cells supernatant on SDS-PAGE gel.

(Lane 1) Prior to the purification of supernatant. (Lane 2) Flow- through. (Lane 3) Unstained Protein MW Marker (Thermo Scientific). (Lane 4–10) The fractions of second elution buffer. The fractions of first elution buffer was cropped. The full-length gel is presented in Supplementary Figure S1.