Figure 1: Effects of Ac-PGP on the migration, tube formation, and proliferation activities of hEPCs. | Scientific Reports

Figure 1: Effects of Ac-PGP on the migration, tube formation, and proliferation activities of hEPCs.

From: N-Acetylated Proline-Glycine-Proline Accelerates Cutaneous Wound Healing and Neovascularization by Human Endothelial Progenitor Cells

Figure 1

(a) Migration of hEPCs was measured using a chemotaxis chamber in response to VEGF (10 ng/ml) or various concentrations of Ac-PGP after 12 hour incubation. Data indicate mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 versus control (n = 6). (b) Representative images of tube formation in response to Ac-PGP (0.1 μM) or VEGF (10 ng/ml) are shown (upper panels). Tube formation was quantified by measuring the length of tubes (lower panel). Data indicate mean ± SD. *p < 0.05, **p < 0.01, versus control (n = 6). Bar = 50 μm. (c) The proliferative effect of Ac-PGP on hEPCs, hEPCs was measured by staining with anti-PCNA antibody (green). Nuclei were counterstained with DAPI (blue). Numbers of PCNA-positive nuclei per field were counted and expressed as the relative percentage of total cells. Data indicate mean ± SD. **p < 0.01, ***p < 0.001 versus control (n = 6). Bar = 100 μm.

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