Figure 2: Role of CXCR2 in the Ac-PGP-induced migration and tube formation of hEPCs. | Scientific Reports

Figure 2: Role of CXCR2 in the Ac-PGP-induced migration and tube formation of hEPCs.

From: N-Acetylated Proline-Glycine-Proline Accelerates Cutaneous Wound Healing and Neovascularization by Human Endothelial Progenitor Cells

Figure 2

(a) hEPCs were transfected with control siRNA (si-control) or CXCR2 specific siRNA (si-CXCR2), and mRNA levels of CXCR2 and GAPDH were determined by reverse transcription-polymerase chain reaction. (b) Silencing of CXCR2 abrogates Ac-PGP (0.1 μM)-stimulated migration of hEPCs but not VEGF-stimulated migration. Data indicate mean ± SD. **p < 0.01, ***p < 0.001 versus control, ##p < 0.01, si-CXCR2 VEGF versus si-CXCR2 control (n = 8) (c) Representative images of tube formation by hEPCs in response to Ac-PGP (0.1 μM) or VEGF (10 ng/ml) with or without CXCR2 knockdown are shown (left panels). Quantitative analysis of tube length is shown in the right panel. Data indicate mean ± SD. *p < 0.5, versus control, #p < 0.05, si-CXCR2 VEGF versus si-CXCR2 control (n = 4). Bar = 50 μm.

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